STRUCTURE AND DEVELOPMENTAL REGULATION OF THE MURINE CTK GENE

被引：9

作者：

BRINKLEY, PM ^{[1
]}

CLASS, K ^{[1
]}

BOLEN, JB ^{[1
]}

PENHALLOW, RC ^{[1
]}

机构：

[1] BRISTOL MYERS SQUIBB PHARMACEUT RES INST,DEPT MOLEC BIOL,PRINCETON,NJ 08543

来源：

GENE | 1995年 / 163卷 / 02期

关键词：

PROTEIN TYROSINE KINASE; ALTERNATE EXON USAGE; MOUSE BRAIN; RNASE PROTECTION SRC; PRIMER EXTENSION; WESTERN BLOT;

D O I：

10.1016/0378-1119(95)00352-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Csk-type protein tyrosine kinase (Ctk) is a protein tyrosine kinase with structural and functional similarities to C-terminal Src kinase (Csk). These enzymes catalyze the phosphorylation of C-terminal regulatory tyrosine residues of Src family enzymes. Here we describe the structure of the ctk gene and characterize the pattern of ctk expression, Ctk and a closely related enzyme, nervous tissue and T-lymphocyte kinase (Ntk), are assembled by selective use of two of the exons. Expression of ctk was found to be restricted primarily to the brain. ctk and csk exhibit a different expression profile in the developing mouse brain. The expression of the ctk mRNA and mature polypeptide increase postnatally, while the expression of csk decreases with age. Our results thus show that despite their structural and functional similarities, ctk and csk differ markedly in their patterns of expression.

引用

页码：179 / 184

页数：6

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