INVIVO PHOSPHORYLATION OF THE 30-KDA PROTEIN OF TOBACCO MOSAIC-VIRUS

被引：54
|
作者：
WATANABE, Y [1 ]
OGAWA, T [1 ]
OKADA, Y [1 ]
机构：
[1] KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,PCC TECHNOL INC,TOCHIGI LAB,TOCHIGI 32914,JAPAN
来源：
FEBS LETTERS | 1992年 / 313卷 / 02期
关键词：
30-KDA PROTEIN; PHOSPHORYLATION; CELL-TO-CELL MOVEMENT; INVITRO TRANSCRIPT; TOBACCO MOSAIC VIRUS; TOBACCO PROTOPLAST;
D O I：
10.1016/0014-5793(92)81440-W
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The 30-kDa protein of tobacco mosaic virus, which is involved in cell-to-cell movement function, is phosphorylated in tobacco protoplasts. To investigate which portion of the protein is phosphorylated we inoculated several truncated 30-kDa protein mutants into protoplasts and determined whether or not those truncated proteins are phosphorylated. The results showed that amino acid residues 234-261 of the 30-kDa protein are required for this phosphorylation.
引用
收藏
页码：181 / 184
页数：4
相关论文
共 50 条
  • [1] INFLUENCE OF THE TOBACCO MOSAIC-VIRUS 30-KDA MOVEMENT PROTEIN ON CARBON METABOLISM AND PHOTOSYNTHATE PARTITIONING IN TRANSGENIC TOBACCO PLANTS
    LUCAS, WJ
    OLESINSKI, A
    HULL, RJ
    HAUDENSHIELD, JS
    DEOM, CM
    BEACHY, RN
    WOLF, S
    PLANTA, 1993, 190 (01) : 88 - 96
  • [2] Regulation of plasmodesmal transport by phosphorylation of tobacco mosaic virus cell-to-cell movement protein
    Waigmann, E
    Chen, MH
    Bachmaier, R
    Ghoshroy, S
    Citovsky, V
    EMBO JOURNAL, 2000, 19 (18) : 4875 - 4884
  • [3] VIRUS-RESISTANCE OF TRANSGENIC TOBACCO PLANTS EXPRESSING TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE
    TIAN, YC
    QIN, XF
    WANG, GL
    YANG, MZ
    FANG, RX
    XU, BY
    MANG, KQ
    SCIENCE IN CHINA SERIES B-CHEMISTRY, 1991, 34 (11): : 1329 - 1340
  • [4] DEVELOPMENTAL-CHANGES IN PLASMODESMATA IN TRANSGENIC TOBACCO EXPRESSING THE MOVEMENT PROTEIN OF TOBACCO MOSAIC-VIRUS
    MOORE, PJ
    FENCZIK, CA
    DEOM, CM
    BEACHY, RN
    PROTOPLASMA, 1992, 170 (3-4) : 115 - 127
  • [5] Phosphorylation of the Movement Protein of Cucumber Mosaic Virus in Transgenic Tobacco Plants
    Yasuhiko Matsushita
    Kuniaki Yoshioka
    Takuma Shigyo
    Hideki Takahashi
    Hiroshi Nyunoya
    Virus Genes, 2002, 24 : 231 - 234
  • [6] Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants
    Matsushita, Y
    Yoshioka, K
    Shigyo, T
    Takahashi, H
    Nyunoya, H
    VIRUS GENES, 2002, 24 (03) : 231 - 234
  • [7] THE MECHANISM(S) OF COAT PROTEIN-MEDIATED RESISTANCE AGAINST TOBACCO MOSAIC-VIRUS
    REIMANNPHILIPP, U
    BEACHY, RN
    SEMINARS IN VIROLOGY, 1993, 4 (06): : 349 - 356
  • [8] SCANNING CALORIMETRIC STUDIES OF THE STABILITY OF TOBACCO MOSAIC-VIRUS AND AGGREGATES OF ITS COAT PROTEIN
    MUTOMBO, K
    MICHELS, B
    OTT, H
    CERF, R
    WITZ, J
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 1992, 21 (01): : 77 - 83
  • [9] PRODUCTION OF ENKEPHALIN IN TOBACCO PROTOPLASTS USING TOBACCO MOSAIC-VIRUS RNA VECTOR
    TAKAMATSU, N
    WATANABE, Y
    YANAGI, H
    MESHI, T
    SHIBA, T
    OKADA, Y
    FEBS LETTERS, 1990, 269 (01) : 73 - 76
  • [10] PHOSPHORYLATION OF TOBACCO MOSAIC-VIRUS CELL-TO-CELL MOVEMENT PROTEIN BY A DEVELOPMENTALLY REGULATED PLANT-CELL WALL-ASSOCIATED PROTEIN-KINASE
    CITOVSKY, V
    MCLEAN, BG
    ZUPAN, JR
    ZAMBRYSKI, P
    GENES & DEVELOPMENT, 1993, 7 (05) : 904 - 910
12345