CLONING OF XYLANASE GENE OF STREPTOMYCES-FLAVOGRISEUS IN ESCHERICHIA-COLI AND BACTERIOPHAGE LAMBDA-INDUCED LYSIS FOR THE RELEASE OF CLONED ENZYME

被引：0
作者：
SRIVASTAVA, R
ALI, SS
SRIVASTAVA, BS
机构：
来源：
FEMS MICROBIOLOGY LETTERS | 1991年 / 78卷 / 2-3期
关键词：
STREPTOMYCES-FLAVOGRISEUS; ESCHERICHIA-COLI; BACTERIOPHAGE-LAMBDA; XYLANASE GENE;
D O I：
10.1016/0378-1097(91)90158-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda-cI857. Lambda-Induced lysis of E. coli at 42-degrees-C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII fragment and coded for 18 000 M(r) xylanase.
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页码：201 / 206
页数：6
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