STEROIDOGENESIS IN PORCINE FOLLICLE CELLS CULTURED IN SERUM-FREE MEDIA

被引：3
|
作者：
STEIN, AL [1 ]
DIZEREGA, GS [1 ]
TONETTA, SA [1 ]
机构：
[1] UNIV SO CALIF, SCH MED,DEPT OBSTET & GYNECOL, LIVINGSTON REPROD BIOL LAB,1321 N MISS RD, LOS ANGELES, CA 90033 USA
来源：
GYNECOLOGIC AND OBSTETRIC INVESTIGATION | 1990年 / 29卷 / 04期
关键词：
Granulosa cells; Ovary; Steroidogenesis; Theca cells;
D O I：
10.1159/000293336
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
The purpose of this study was to determine the steroid production of cultured porcine follicle cells grown in serum-free media. Theca cells (TC) and granulosa cells (GC) from large porcine follicles (greater than 8 mm) were dispersed and plated as monolayer cultures in serum-free media. Media were removed from the cultures at 3, 6, 12, 24, 48, 72, 96, and 120 h and assayed for estradiol, progesterone, testosterone and androstenedione. Both GC and TC were capable of producing progesterone, androgens and estradiol in serum-free media. GC were responsive to human chorionic gonadotropin and follicle-stimulating hormone while TC were only responsive to human chorionic gonadotropin. Growth factors, particularly insulin, appeared to enhance ovarian cells’ steroidogenesis. © 1990 S. Karger AG, Basel.
引用
收藏
页码：285 / 288
页数：4
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