STUDIES ON THYROXINE-BINDING GLOBULIN (TBG) .2. SEPARATION FROM HUMAN SERUM BY AFFINITY CHROMATOGRAPHY

Thyroxine-binding globulin (TBG) has been separated from human serum by affinity chromatography using Sepharose to which l-thyroxine had been covalently attached. Further purification of the TBG in the material eluted from the T4-Sepharose was carried out by DEAE-Sephadex column chromatography. The final product produced a single stained band on analytic disc-gel electrophoresis. Radio-autography of this material to which tracer amounts of radioactive thyroxine had been added prior to electrophoresis on cellulose acetate showed a single band of thyroxine radioactivity with the electrophoretic mobility of TBG in normal human serum. The calculated yield of TBG by this procedure was 18-37%. © 1969.