CHROMATOGRAPHIC SEPARATION OF ESTRONE AND 17BETA-ESTRADIOL CONJUGATES ON DEAE-SEPHADEX

17β-Estradiol-3-sulf ate (E2 3S), 17β-estradiol-17-sulfate (E2 17S), 17β-estradiol-3-sulfate-17-glucosiduronate(E23S17G) and 17β-estradiol-3, 17-disulfate (E2 3S17S), all labelled with 3H, were prepared in the laboratory and identified by chromatographic, hydrolytic and crystallization techniques. Estrone-3-glucosiduronate(E1 3G) and 17β-estradiol-3-glucosiduronate (E23G), both labelled with 14C, were also synthesized and similarly identified. Column chromatography on DEAE-Sephadex in a linear gradient of 0-0.8M NaCl, followed by 0.8-2.0M NaCl, resulted in a clear separation of 17β-estradiol(E2), E1 3G, 17β-estradiol-17-glucosiduronate (E217G), estrone-S-sulfatetE1 3S), E23S, E2 3S17G and E2 3S17S. E23S was not separable from E217S under these conditions. The presence of urinary residues, while causing some changes in the elution pattern, still allowed of a reasonable separation. Some resolution of E13G and E23G was possible in a linear gradient (0-0.4M NaCl). Also, some separation could be obtained between E23G and E217G in a non-linear gradient (concave upwards; 0-1.OM NaCl). © 1968.