SOME FACTORS DETERMINING PROTEIN AGGREGATION DURING ULTRAFILTRATION

被引：64

作者：

KIM, KJ ^{[1
]}

CHEN, V ^{[1
]}

FANE, AG ^{[1
]}

机构：

[1] UNIV NEW S WALES,SCH CHEM ENGN & IND CHEM,CTR MEMBRANE SCI & TECHNOL,KENSINGTON,NSW 2033,AUSTRALIA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1993年 / 42卷 / 02期

关键词：

FOULING; ULTRAFILTRATION; PROTEIN AGGREGATES; FIELD EMISSION SCANNING ELECTRON MICROSCOPY (FESEM);

D O I：

10.1002/bit.260420216

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The factors contributing to protein aggregation in albumin ultrafiltration were investigated as a function of operation conditions. The nature of protein deposits was examined by electron microscopy. Protein aggregation appears to occur as a result of rapid supersaturation of protein molecules and high solvent velocity (shear) in the concentrated layer near the membrane surface. The shear occurring in the solvent flow on the membrane surface probably unfolds protein molecules and thus promotes flocculation due to collision between particles.

引用

页码：260 / 265

页数：6

共 9 条

[1] PROPERTIES OF MICROFILTRATION MEMBRANES - FLUX LOSS DURING CONSTANT PRESSURE PERMEATION OF BOVINE SERUM-ALBUMIN [J].