CLONING, EXPRESSION AND CHARACTERIZATION OF HORSE L-FERRITIN IN ESCHERICHIA-COLI

被引：42
|
作者：
TAKEDA, S
OHTA, M
EBINA, S
NAGAYAMA, K
机构：
[1] Protein Array Project, ERATO, JRDC, Tsukuba
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 02期
关键词：
FERRITIN; RECOMBINANT DNA; AMINO ACID SEQUENCE; (HORSE);
D O I：
10.1016/0167-4781(93)90121-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Horse L-ferritin cDNA was cloned from horse liver, and the base sequence was determined. The L-ferritin was expressed using pTZ18U encoding lac promoter, and found to possess an additional 8-amino acid sequence at the N-terminus as compared with commercially obtained horse spleen (natural) ferritin. It was determined that there was Pro at position 94 in both the recombinant and natural L-ferritin, although it was previously reported that Leu was in this position in the natural species. Transmission electron microscopy showed that this recombinant ferritin formed a 24-mer shell.
引用
收藏
页码：218 / 220
页数：3
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