INVESTIGATIONS OF RETENTION RULE IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - THEORETICAL CONSIDERATION OF RETENTION CONVERGENCE AND EXPERIMENTAL-VERIFICATION

This paper studied the effect of different chromatographic parameters in RP-HPLC (for example, composition of mobile phase, temperature, number of carbon atoms within the solute molecules, solvent molecules and alkyl ligand on bonded phase surface) on the retention convergence based on the displacement adsorption multi-interaction model, and derived a few retention convergent equations by using thermodynamic method, which have been verified by a great deal of experimental data of homologous series. Moreover, we have developed a general method, which can calculate the coordinate values of various retention convergent points by computer directly from experimental data, and the results predicted are in good agreement with that obtained by using plotting method.