ANTISENSE DNA OLIGONUCLEOTIDES .1. THE USE OF IONSPRAY TANDEM MASS-SPECTROMETRY FOR THE SEQUENCE VERIFICATION OF METHYLPHOSPHONATE OLIGODEOXYRIBONUCLEOTIDES

被引：37

作者：

BAKER, TR ^{[1
]}

KEOUGH, T ^{[1
]}

DOBSON, RLM ^{[1
]}

RILEY, TA ^{[1
]}

HASSELFIELD, JA ^{[1
]}

HESSELBERTH, PE ^{[1
]}

机构：

[1] JBL SCI INC,SAN LUIS OBISPO,CA 93401

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 03期

关键词：

D O I：

10.1002/rcm.1290070305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequences of synthetically prepared methylphosphonate oligodeoxyribonucleotides have been verified using ionspray tandem mass spectrometry with sample introduction via flow injection. The technique involves the use of product-ion scans from multiply protonated (4+ and 5+) precursors. Among the ions detected are several series of fragments of different charge states that indicate the base sequence of the intact molecule. Oligomers as large as 18 bases have been successfully characterized.

引用

页码：190 / 194

页数：5

共 18 条

[1] FAST-ATOM-BOMBARDMENT COMBINED WITH TANDEM MASS-SPECTROMETRY FOR DETERMINING STRUCTURES OF SMALL OLIGONUCLEOTIDES [J].