DETERMINATION OF DIFFUSION COEFFICIENTS BY SPREADING FROM THIN LAYERS

被引:9
作者
POLSON, A
PARKYN, DG
机构
[1] C.S.I.R. and U.C.T. Virus Research Unit, Medical School, Cape Town
[2] Department of Applied Mathematics, U.C.T, Cape Town
关键词
D O I
10.1002/bip.1969.360070111
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two new methods for the measurement of diffusion coefficients were introduced. In the first method the spreading is measured from a. thin layer in a cylindrical tube containing a suitable gel. To avoid convection in the gel the tube is rotated horizontally round its long axis. In the second method the diffusion spreading is measured from an initial thin layer under a column of solvent in a Tiselius electrophoresis cell or a micro cuvet of a spectrophotometer. The great advantage is the economy on the material investigated. Copyright © 1969 John Wiley & Sons, Inc.
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页码:107 / &
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