FUNCTIONAL-CHARACTERIZATION OF RAT CHEMOKINE MACROPHAGE INFLAMMATORY PROTEIN-2

被引：95
作者：
FREVERT, CW [1 ]
FARONE, A [1 ]
DANAEE, H [1 ]
PAULAUSKIS, JD [1 ]
KOBZIK, L [1 ]
机构：
[1] BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA
来源：
INFLAMMATION | 1995年 / 19卷 / 01期
关键词：
D O I：
10.1007/BF01534386
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50 mu g of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models.
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页码：133 / 142
页数：10
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