A COMPARATIVE-EVALUATION OF THE METABOLIC PROFILES OF NORMAL AND INFLAMMATORY KNEE-JOINT SYNOVIAL-FLUIDS BY HIGH-RESOLUTION PROTON NMR-SPECTROSCOPY

被引：73

作者：

NAUGHTON, DP

HAYWOOD, R

BLAKE, DR

EDMONDS, S

HAWKES, GE

GROOTVELD, M

机构：

[1] UNIV LONDON LONDON HOSP,COLL MED,INFLAMMAT RES GRP,ARC BLDG,25-29 ASHFIELD ST,LONDON E1 2AD,ENGLAND

[2] QUEEN MARY & WESTFIELD COLL,DEPT CHEM,LONDON,ENGLAND

来源：

FEBS LETTERS | 1993年 / 332卷 / 03期

关键词：

NORMAL SYNOVIAL FLUID; H-1 NMR SPECTROSCOPY; INFLAMMATORY JOINT DISEASE; HYPOXIA; LOW-DENSITY-LIPOPROTEIN; ACUTE-PHASE GLYCOPROTEIN;

D O I：

10.1016/0014-5793(93)80636-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High resolution H-1 NMR spectroscopy has been employed to investigate the metabolic profile of heatlhy human knee-joint synovial fluid (SF) and the biochemical data acquired have been compared with those of matched serum, and inflammatory knee-joint SF samples. Results obtained indicate that the healthy human knee-joint has a hypoxic status (high lactate level when expressed relative to that of paired serum) that is milder than that of the inflamed human knee-joint. Moreover, normal SF differs from that of inflammatory SF in that it contains little or no NMR-detectable lipoprotein-associated fatty acids and 'acute-phase' glycoproteins, an observation reflecting the limited passage of these macromolecules from plasma into the synovial space in healthy subjects.

引用

页码：221 / 225

页数：5

共 15 条

[1]

Bell J D, 1989, NMR Biomed, V2, P246, DOI 10.1002/nbm.1940020513

[2] ASSIGNMENT OF RESONANCES FOR ACUTE-PHASE GLYCOPROTEINS IN HIGH-RESOLUTION PROTON NMR-SPECTRA OF HUMAN-BLOOD PLASMA [J].