MOLECULAR-CLONING AND CHARACTERIZATION OF HUMAN ENDOTHELIAL NITRIC-OXIDE SYNTHASE

被引：428

作者：

MARSDEN, PA

SCHAPPERT, KT

CHEN, HS

FLOWERS, M

SUNDELL, CL

WILCOX, JN

LAMAS, S

MICHEL, T

机构：

[1] UNIV TORONTO,ST MICHAELS HOSP,DEPT MED,TORONTO M5B 1W8,ONTARIO,CANADA

[2] UNIV TORONTO,ST MICHAELS HOSP,DIV RENAL,TORONTO M5B 1W8,ONTARIO,CANADA

[3] UNIV TORONTO,HOSP SICK CHILDREN,DEPT GENET,TORONTO M5G 1X8,ONTARIO,CANADA

[4] EMORY UNIV,DEPT MED,ATLANTA,GA 30322

[5] HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DIV CARDIOVASC,BOSTON,MA 02115

来源：

FEBS LETTERS | 1992年 / 307卷 / 03期

关键词：

CYCLIC GMP; ENDOTHELIUM-DERIVED RELAXING FACTOR; ENDOTHELIUM; VASCULAR SMOOTH MUSCLE; VASODILATATION;

D O I：

10.1016/0014-5793(92)80697-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The constitutive calcium/calmodulin-dependent nitric oxide (NO) synthase expressed in vascular endothelium shares common biochemical and pharmacologic properties with neuronal NO synthase. However, recent cloning and molecular characterization of NO synthase from bovine endothelial cells indicated the existence of a family of constitutive NO synthases. Accordingly, we undertook molecular cloning and sequence analysis of human endothelial NO synthase. Complementary DNA clones predict a protein of 1,203 amino acids sharing 94% identity with the bovine endothelial protein, but only 60% identity with the rat brain NO synthase isoform. Northern blot analysis with an endothelial-derived cDNA identified a 4.6-4.8 kb mRNA transcript in HUVEC and in situ hybridization localized transcripts to vascular endothelium but not neuronal tissue.

引用

页码：287 / 293

页数：7

共 22 条

[1] INTERLEUKIN-1 INDUCES PROLONGED L-ARGININE-DEPENDENT CYCLIC GUANOSINE-MONOPHOSPHATE AND NITRITE PRODUCTION IN RAT VASCULAR SMOOTH-MUSCLE CELLS [J].