INFLUENCE OF AGE ON THE RHYTHM IN BASAL AND FORSKOLIN-STIMULATED ADENYLATE-CYCLASE ACTIVITY OF THE RAT-HEART

被引：6

作者：

LEMMER, B

WALD, C

机构：

[1] Zentrum der Pharmakologie, J.W. Goethe-Universitat, D-6000, Frankfurt/M

来源：

CHRONOBIOLOGY INTERNATIONAL | 1990年 / 7卷 / 02期

关键词：

Age-dependency; Forskolin; Key words-Adenylate cyclase; Rat heart; Rhythmicity;

D O I：

10.3109/07420529009056962

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In male rats of three different ages (Group 1:7 weeks, Group II: 4 months, Group III: ≥ 10 months) basal and forskolin-stimulated adenylate cyclase (AC) activity were investigated in rat heart ventricles of animals sacrificed at 8 time points within 24 hours of a day. Maximum (Emax) and half-maximum (EC50) stimulation by a water-soluble forskolin (FOR; 0.1-100 μmol/l) were determined from dose-response curves. In young rats (Group I) significant rhythmicity was found in basal and FOR-stimulated AC activity, which was successively reduced or abolished with age by a reduction in amplitude. Mean basal AC activity increased about 2-fold from Group I to Group II/III. Mean maximum in for stimulation was about 12-fold in Group I and about 7-fold in Group II and ID. EC50-values displayed neither a significant rhythmicity in either group nor an age-dependency. It is concluded that the stimulatory potency of the catalytic subunit of the AC is reduced with age. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：107 / 111

页数：5

共 10 条

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