Membrane Stabilizing and Antioxidant Activity of Ougeinia oojeinensis Seed Extracts and Their Fatty Acid Composition

In the present study different extracts of seeds of Ougeinia oojeinensis were prepared and evaluated their membrane stabilizing and antioxidant effects. The Fatty acid composition was also estimated. All extract were tested for presence of phytoconstituents i.e., alkaloid, carbohydrate, sterols, proteins, amino acids, saponin, and phenolic compounds in different extracts. Membrane stabilizing effect was studied by hypotonic solution induced haemolysis of erythrocyte. Antioxidant activity was studied by DPPH method at a different concentration. GCMS analysis was done for petroleum ether extract with the help of Perkin Elmer Clarus-500 model coupled with CLARUS-500 Mass spectrometer. Phytochemical analysis showed that methanol extract was the richest extract for the tested phytoconstituents. Methanol extract showed the presence of alkaloid, carbohydrate, saponin, protein, amino acids and phenolic compounds. Different fatty acids were present in petroleum ether extract which was analyzed by GCMS. Maximum membrane stabilizing activity of seeds of Ougeinia oojeinensis showed in Methanol extract (81.41 +/- 1.28) at a concentration of 1000 mu g/ml in comparison to standard drug aspirin. From antioxidant studies, methanol extract showed maximum antioxidant activity (91.31 +/- 1.31) at a concentration of 1000 mu g/ml than other extract in comparison to standard drug ascorbic acid. From above studies it could be concluded that methanol extract showed maximum membrane stabilizing and antioxidant activities.