IS HYDROGEN-PEROXIDE INVOLVED IN THE BENZYL VIOLOGEN-MEDIATED INVIVO INACTIVATION OF RAT-LIVER GLUTAMINE-SYNTHETASE

被引：0

作者：

MURIANA, FJG ^{[1
]}

RUIZGUTIERREZ, V ^{[1
]}

RELIMPIO, AM ^{[1
]}

机构：

[1] UNIV SEVILLA, FAC FARM, DEPT BIOQUIM, E-41012 SEVILLE, SPAIN

来源：

INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY | 1993年 / 74卷 / 02期

关键词：

BENZYL VIOLOGEN; GLUTAMINE SYNTHETASE; RAT LIVER;

D O I：

暂无

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

After benzyl viologen administration to rats, a decrease in the rat liver glutamine synthetase activity was observed. An increase in the rat liver catalase activity was found concomitantly. In combination with the catalase inhibitor aminotriazole, benzyl viologen again diminished, but markedly, the rat liver glutamine synthetase activity. Moreover, partially purified glutamine synthetase from rat liver underwent rapid inactivation upon aerobic incubation with NAD(P)H and benzyl viologen. This inactivation was prevented by catalase, which suggests that the NAD(P)H/BV2+/O2-dependent system has a role in H2O2 production. Our results suggest that H2O2 is involved in the benzyl viologen-mediated in-vivo inactivation of the rat liver glutamine synthetase. In contrast, benzyl viologen alone or in combination with aminotriazole produced a significant increase of brain glutamine synthetase.

引用

页码：219 / 224

页数：6

共 33 条

[1] Aebi H, 1974, METHOD ENZYMAT AN, P673, DOI DOI 10.1016/B978-0-12-091302-2.50032-3
[2] BERL S, 1962, J BIOL CHEM, V237, P2562
[3] CYTOTOXIC REACTIONS OF FREE-RADICAL SPECIES OF OXYGEN
BORG, DC
SCHAICH, KM
ELMORE, JJ
BELL, JA
[J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1978, 28 (4-5) : 887 - 907
[4] INCREASE IN HEPATIC MIXED DISULFIDE AND GLUTATHIONE DISULFIDE LEVELS ELICITED BY PARAQUAT
BRIGELIUS, R
LENZEN, R
SIES, H
[J]. BIOCHEMICAL PHARMACOLOGY, 1982, 31 (08) : 1637 - 1641
[5] Calderbank A, 1968, Adv Pest Control Res, V8, P127
[6] DEUEL TF, 1978, J BIOL CHEM, V253, P6111
[7] ACTION OF PARAQUAT AND DIQUAT ON RESPIRATION OF LIVER CELL FRACTIONS
GAGE, JC
[J]. BIOCHEMICAL JOURNAL, 1968, 109 (05) : 757 - +
[8] STUDIES ON MECHANISM OF LUNG TOXICITY OF PARAQUAT - COMPARISON OF TISSUE DISTRIBUTION AND SOME BIOCHEMICAL PARAMETERS IN RATS AND RABBITS
ILETT, KF
STRIPP, B
MENARD, RH
REID, WD
GILLETTE, JR
[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 1974, 28 (02) : 216 - 226
[9] JONES GL, 1982, BIOCHIM BIOPHYS ACTA, V712, P420, DOI 10.1016/0005-2760(82)90362-9
[10] INHIBITION OF GLUTAMINE-SYNTHETASE ACTIVITY BY MANGANOUS IONS IN A CYTOSOL EXTRACT OF RAT-LIVER
JOSEPH, SK
BRADFORD, NM
MCGIVAN, JD
[J]. BIOCHEMICAL JOURNAL, 1979, 184 (02) : 477 - 480

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