PRODUCTION OF 15-HYDROXY-9-OCTADECANOIC 16-HYDROXY-9-OCTADECANOIC AND 17-HYDROXY-9-OCTADECANOIC ACIDS BY BIOCONVERSION OF OLEIC-ACID WITH BACILLUS-PUMILUS
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作者:
LANSER, AC
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机构:Oil Chemical Research and Bioactive Constituents Research, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peroria, 61604, IL
LANSER, AC
PLATTNER, RD
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机构:Oil Chemical Research and Bioactive Constituents Research, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peroria, 61604, IL
PLATTNER, RD
BAGBY, MO
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机构:Oil Chemical Research and Bioactive Constituents Research, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peroria, 61604, IL
BAGBY, MO
机构:
[1] Oil Chemical Research and Bioactive Constituents Research, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peroria, 61604, IL
Several Bacillus strains were tested for their ability to hydroxylate oleic acid. Two strains-BD-174 and BD-226-converted oleic acid into a trio of hydroxy-octadecenoic acids. Bioconversion in 72-120 hr produced 5-11% of hydroxy acids relative to oleic acid as measured by gas chromatography. These acids were identified as the 15-, 16- and 17-hydroxy-9-octadecenoic acids by gas chromatography-mass spectrometry of trimethyl silyl derivatives of the product acids and their hydrogenated counterparts.