THE CELLOBIOHYDROLASE-I FROM TRICHODERMA-REESEI QM-9414 - ACTION ON CELLO-OLIGOSACCHARIDES

被引：73
|
作者：
VRSANSKA, M [1 ]
BIELY, P [1 ]
机构：
[1] SLOVAK ACAD SCI, INST CHEM, CS-84238 BRATISLAVA, CZECHOSLOVAKIA
来源：
CARBOHYDRATE RESEARCH | 1992年 / 227卷
关键词：
D O I：
10.1016/0008-6215(92)85058-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The action of cellobiohydrolase I [(1 --> 4)-beta-D-glucan cellobiohydrolase, EC 3.2.1.91, CBH-I] from Trichoderma reesei QM 9414 on cello-oligosaccharides labelled with tritium in the reducing moiety has been investigated. There was a marked preference of CBH-I to attack the first three linkages at the reducing ends of the cello-oligosaccharides (cellotriose-cellohexaose). [1-H-3]Cellobiose and 4-methylumbelliferyl beta-D-glucopyranoside served as glycosyl acceptors during the degradation of cellotriose by CBH-I.
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页码：19 / 27
页数：9
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