MAGNETIC DNA HYBRIDIZATION PROPERTIES OF OLIGONUCLEOTIDE PROBES ATTACHED TO SUPERPARAMAGNETIC BEADS AND THEIR USE IN THE ISOLATION OF POLY(A) MESSENGER-RNA FROM EUKARYOTIC CELLS

被引：49

作者：

HORNES, E

KORSNES, L

机构：

[1] Apothekernes Laboratorium A.S., Oslo

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 06期

关键词：

D O I：

10.1016/0735-0651(90)90028-E

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(A) messenger RNA is generally purified from total RNA using oligo(dT) cellulose affinity chromatography or centrifugation through spin columns. We present a new method for rapid purification of poly(A) mRNA using oligo(dT) probes attached to superparamagnetic beads. By magnetic separation, washing, and elution, pure mRNA is obtained from living cells within 10 minutes. This procedure works for crude RNA preparations or cell lysates that would otherwise clog standard oligo(dT) cellulose column systems. The present method reduces the risk of degradation, is highly efficient, and can easily be scaled up or down.

引用

页码：145 / 150

页数：6

共 9 条

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[2]

Ausubel FM, 2003, CURRENT PROTOCOLS MO

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