CRYSTALLIZATION OF HUMAN TUMOR-NECROSIS-FACTOR

被引:5
作者
WALKER, N
MARCINOWSKI, S
HILLEN, H
MACHTLE, W
JONES, Y
STUART, D
机构
[1] BASF AG,KUNSTOFFLAB,W-6700 LUDWIGSHAFEN,GERMANY
[2] LAB MOLEC BIOPHYS,OXFORD OX1 3QU,ENGLAND
关键词
D O I
10.1016/0022-0248(90)90619-V
中图分类号
O7 [晶体学];
学科分类号
0702 ; 070205 ; 0703 ; 080501 ;
摘要
Crystals of recombinant human tumour necrosis factor, an 157 amino acid protein expressed in Escherichia coli, have been obtained that are suitable for a full structure determination. A dramatic increase in crystal size was obtained with the addition of the non-ionic detergent β-octyl glucoside. The crystals belong to the trigonal space group P3121 with unit cell dimensions a=b=166.0 å and c=93.0 å, and diffract to 2.9 å. Crystal density measurements indicated a solvent content of close to 65%. Since TNF has been proposed to exist at the trimer in solution, this is consistent with an arrangement of two independent trimers, i.e. six molecules, in the asymmetric unit. © 1990.
引用
收藏
页码:168 / 170
页数:3
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