THIAISOLEUCINE AND PROTEIN-SYNTHESIS

被引：9

作者：

BUSIELLO, V ^{[1
]}

DIGIROLAMO, M ^{[1
]}

DEMARCO, C ^{[1
]}

机构：

[1] UNIV ROME,CNR,CTR BIOL MOLEC,IST CHIM BIOL,I-00100 ROME,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 561卷 / 01期

关键词：

Isoleucine tRNA; Protein synthesis; Thiaisoleucine;

D O I：

10.1016/0005-2787(79)90503-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thiaisoleucine is an isoleucine analogue having the γ-methylene group of the valerianic carbon chain substituted by a sulphur atom. It has been demonstrated that thiaisoleucine is activated and transferred to tRNAIle by rat liver aminoacyl-tRNA synthetase and inhibits isoleucine incorporation into polypeptides in protein synthesizing systems from rat liver or rabbit reticulocytes, whereas it does not affect either leucine incorporation or ribosome run-off or polypeptide chain elongation rate. All tests were performed in comparison with O-methyl-threonine, an isoleucine analogue with the γ-methylene group substituted by an oxygen atom. In all the reactions studied, both thiaisoleucine and O-methyl-threonine act as competitive inhibitors of isoleucine. With respect to O-methyl-threonine, thiaisoleucine shows higher activity as an isoleucine inhibitor. © 1979.

引用

页码：206 / 214

页数：9

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