IMMOBILIZATION OF BETA-GALACTOSIDASES FROM THERMUS-AQUATICUS YT-1 FOR OLIGOSACCHARIDES SYNTHESIS

被引：17

作者：

BERGER, JL

LEE, BH

LACROIX, C

机构：

[1] MCGILL UNIV,DEPT FOOD SCI,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA

[2] AGR CANADA,FRDC,ST HYACINTHE,PQ J2S 8E3,CANADA

来源：

BIOTECHNOLOGY TECHNIQUES | 1995年 / 9卷 / 08期

关键词：

D O I：

10.1007/BF00152452

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

beta-galactosidases of Thermus aquaticus YT-1, exhibiting a galactosyl transferase activity, were immobilized using different techniques. Entrapment in agarose or gellan gum beads was unsuitable for enzyme immobilization due to enzyme leakage. A technique that efficiently immobilized the enzymes was developed using glutaraldehyde co-crosslinking of beta-galactosidases with bovine serum albumin, followed by entrapment in agarose beads.

引用

收藏

页码：601 / 606

页数：6

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