HETEROLOGOUS GENE-EXPRESSION AND PROTEIN SECRETION FROM CANDIDA-GLABRATA

被引：0
作者：
MACREADIE, IG
CASTELLI, LA
MEHRA, RK
THORVALDSEN, JL
WINGE, DR
机构：
[1] UNIV UTAH, DEPT BIOCHEM, SALT LAKE CITY, UT 84132 USA
[2] UNIV UTAH, DEPT MED, SALT LAKE CITY, UT 84132 USA
来源：
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 1994年 / 19卷
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have examined heterologous protein secretion from Candida glabrata with the aid of a stable C. glabrata vector and a secretion reporter cassette comprising the Saccharomyces cerevisiae PGK-gene promoter and a Kluveromyces lactis secretion signal to drive secretion of Escherichia coli beta-lactamase. Abundant secretion of beta-lactamase from C. glabrata indicates that the S. cerevisiae PGK promoter functions in C. glabrata. Furthermore, we show that C. glabrata processes the secreted beta-lactamase in a manner similar to, but not identical with, S. cerevisiae and K. lactis. C. glabrata may be a suitable new host for the expression of foreign genes.
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页码：265 / 269
页数：5
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