PENICILLIN-G ACYLASE FROM KLUYVERA-CITROPHILA NEW CHOICE AS INDUSTRIAL ENZYME

被引：30
作者：
ALVARO, G
FERNANDEZLAFUENTE, R
ROSELL, CM
BLANCO, RM
GARCIALOPEZ, JL
GUISAN, JM
机构：
[1] UNIV AUTONOMA MADRID,CSIC,INST CATALISIS,CANTOBLANCO,E-28049 MADRID,SPAIN
[2] CSIC,CTR INVEST BIOL,E-28006 MADRID,SPAIN
来源：
BIOTECHNOLOGY LETTERS | 1992年 / 14卷 / 04期
关键词：
D O I：
10.1007/BF01022325
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We propose a new and integrated method for the evaluation of industrial enzymes. The application of this method to the enzyme penicillin G acylase from Kluyvera citrophila shows very interesting industrial prospects. This acylase presents a much better stability against heat, pH or organic cosolvents as compared with the more popular enzyme from Escherichia coli. In addition, this enzyme is very easy to immobilize through its amine groups and to stabilize through multipoint covalent attachment on activated pre-existing supports.
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页码：285 / 290
页数：6
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