CONFIGURATIONALLY DEFINED PHOSPHOROTHIOATE-CONTAINING OLIGORIBONUCLEOTIDES IN THE STUDY OF THE MECHANISM OF CLEAVAGE OF HAMMERHEAD RIBOZYMES

被引：247

作者：

SLIM, G ^{[1
]}

GAIT, MJ ^{[1
]}

机构：

[1] MRC,MOLEC BIOL LAB,HILLS RD,CAMBRIDGE CB2 2QH,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 06期

关键词：

D O I：

10.1093/nar/19.6.1183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical synthesis is described of oligoribonucleotides containing a single phosphorothioate linkage of defined Rp and Sp configuration. The oligoribonucleotides were used as substrates in the study of the mechanism of cleavage of an RNA hammerhead domain having the phosphorothioate group at the cleavage site. Whereas the Rp isomer was cleaved only very slowly in the presence of magnesium ion, the rate of cleavage of the Sp isomer was only slightly reduced from that of the unmodified phosphodiester. This finding gives further evidence for the hypothesis that the magnesium ion is bound to the pro-R oxygen in the transition state of the hammerhead cleavage reaction. Also, inversion of configuration at phosphorus is confirmed for a two-stranded hammerhead.

引用

页码：1183 / 1188

页数：6

共 36 条

[1] ABSOLUTE-CONFIGURATION OF DIASTEREOMERS OF ADENOSINE 5'-O-(1-THIOTRIPHOSPHATE) - CONSEQUENCES FOR STEREOCHEMISTRY OF POLYMERIZATION BY DNA-DEPENDENT RNA-POLYMERASE FROM ESCHERICHIA-COLI [J].