V-SRC AND EJ RAS ALLEVIATE REPRESSION OF C-JUN BY A CELL-SPECIFIC INHIBITOR

被引:84
作者
BAICHWAL, VR [1 ]
PARK, A [1 ]
TJIAN, R [1 ]
机构
[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720
关键词
D O I
10.1038/352165a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
THE AP-1 family of transcription factors, which includes the proto-oncogene products c-Jun and c-Fos 1, controls the stimulation of cellular genes by growth factors and the expression of oncogenes, including src and ras 2-8. Transcriptional activation by c-Jun is regulated by a cell-type-specific inhibitor that represses the activity of a transcriptional activation domain (A1) of c-Jun by operating through the adjacent negative regulatory region (delta) (refs 9-11). Here we show that cotransfection of the src or ras oncogene enhances the transcriptional activity of a GAL4:c-Jun hybrid that includes the delta-A1 region of c-Jun, suggesting that the DNA binding and dimerization domain of c-Jun is not required for stimulation by Src or Ras. Moreover, induction of c-Jun activity by Src and Ras occurs in cell lines containing the c-Jun inhibitor but not in a cell line lacking it. The region in c-Jun essential for the stimulatory action of these oncogenes maps to domain A1. These findings suggest the existence of signal-transduction pathways that result in an increase in transcriptional activity of c-Jun and AP-1 by disrupting the c-Jun:inhibitor interaction.
引用
收藏
页码:165 / 168
页数:4
相关论文
共 17 条
[1]   THE JUN PROTO-ONCOGENE IS POSITIVELY AUTOREGULATED BY ITS PRODUCT, JUN/AP-1 [J].
ANGEL, P ;
HATTORI, K ;
SMEAL, T ;
KARIN, M .
CELL, 1988, 55 (05) :875-885
[2]   PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR [J].
ANGEL, P ;
IMAGAWA, M ;
CHIU, R ;
STEIN, B ;
IMBRA, RJ ;
RAHMSDORF, HJ ;
JONAT, C ;
HERRLICH, P ;
KARIN, M .
CELL, 1987, 49 (06) :729-739
[3]   CONTROL OF C-JUN ACTIVITY BY INTERACTION OF A CELL-SPECIFIC INHIBITOR WITH REGULATORY DOMAIN-DELTA - DIFFERENCES BETWEEN V-JUN AND C-JUN [J].
BAICHWAL, VR ;
TJIAN, R .
CELL, 1990, 63 (04) :815-825
[4]  
BOHMANN D, 1989, CELL, V59, P909
[5]   EFFICIENT TRANSFORMATION OF CHICKEN-EMBRYO FIBROBLASTS BY C-JUN REQUIRES STRUCTURAL MODIFICATION IN CODING AND NONCODING SEQUENCES [J].
BOS, TJ ;
MONTECLARO, FS ;
MITSUNOBU, F ;
BALL, AR ;
CHANG, CHW ;
NISHIMURA, T ;
VOGT, PK .
GENES & DEVELOPMENT, 1990, 4 (10) :1677-1687
[6]   ONCOGENES AND SIGNAL TRANSDUCTION [J].
CANTLEY, LC ;
AUGER, KR ;
CARPENTER, C ;
DUCKWORTH, B ;
GRAZIANI, A ;
KAPELLER, R ;
SOLTOFF, S .
CELL, 1991, 64 (02) :281-302
[7]   FOS AND JUN - THE AP-1 CONNECTION [J].
CURRAN, T ;
FRANZA, BR .
CELL, 1988, 55 (03) :395-397
[8]   LINKER INSERTION-DELETION MUTAGENESIS OF THE V-SRC GENE - ISOLATION OF HOST-DEPENDENT AND TEMPERATURE-DEPENDENT MUTANTS [J].
DECLUE, JE ;
MARTIN, GS .
JOURNAL OF VIROLOGY, 1989, 63 (02) :542-554
[9]   INDUCTION OF PROTO-ONCOGENE JUN AP-1 BY SERUM AND TPA [J].
LAMPH, WW ;
WAMSLEY, P ;
SASSONECORSI, P ;
VERMA, IM .
NATURE, 1988, 334 (6183) :629-631
[10]   PURIFIED TRANSCRIPTION FACTOR AP-1 INTERACTS WITH TPA-INDUCIBLE ENHANCER ELEMENTS [J].
LEE, W ;
MITCHELL, P ;
TJIAN, R .
CELL, 1987, 49 (06) :741-752