WIDESPREAD DISTRIBUTION OF GQ-ALPHA/G11-ALPHA DETECTED IMMUNOLOGICALLY BY AN ANTIPEPTIDE ANTISERUM DIRECTED AGAINST THE PREDICTED C-TERMINAL DECAPEPTIDE

被引：71

作者：

MITCHELL, FM

MULLANEY, I

GODFREY, PP

ARKINSTALL, SJ

WAKELAM, MJO

MILLIGAN, G

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,MOLEC PHARMACOL GRP,GLASGOW G12 8QQ,SCOTLAND

[2] UNIV GLASGOW,DEPT PHARMACOL,GLASGOW G12 8QQ,SCOTLAND

[3] GLAXO INST MOLEC BIOL,DEPT BIOL CHEM,CH-1228 PLAN LES OUATES,SWITZERLAND

来源：

FEBS LETTERS | 1991年 / 287卷 / 1-2期

关键词：

G-PROTEIN; INOSITOL PHOSPHATE; TRANSMEMBRANE SIGNALING;

D O I：

10.1016/0014-5793(91)80043-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antisera were raised to a synthetic peptide which represents the predicted C-terminal decapeptide of the alpha-subunit of the G-proteins Gq and G11. Competitive ELISA indicated that antiserum CQ2 displayed strong reactivity against this peptide. Antiserum CQ2 identified an apparently single polypeptide of 42 kDa which was expressed widely. The mobility of this polypeptide in SDS-PAGE was not modified by pretreatment of cells with pertussis toxin, indicating that it was not a substrate for this toxin. Furthermore, the levels and mobility of this polypeptide were unaltered by treatment of cells with cholera toxin, defining that it was not related to G(s)alpha.

引用

页码：171 / 174

页数：4

共 24 条

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