GLYCATION-INDUCED CROSS-LINKING OF CALF LENS CRYSTALLINS

被引：24
作者：
VANBOEKEL, MAM
HOENDERS, HJ
机构：
[1] Department of Biochemistry, University of Nijmegen, 6525 EP Nijmegen
来源：
EXPERIMENTAL EYE RESEARCH | 1991年 / 53卷 / 01期
关键词：
GLYCATION; HIGH-MOLECULAR-WEIGHT PROTEIN; CALF LENS; CRYSTALLINS; CROSS-LINKING;
D O I：
10.1016/0014-4835(91)90149-9
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
To investigate the possible role of glycation in the onset of diabetic cataract we used calf lens crystallins as a model. After incubation with reducing sugars, the proteins were investigated by high-pressure gel permeation chromatography, SDS-PAGE and analytical ultracentrifugation. Glucose-6-phosphate incubation resulted in an increase in mean molecular weight of all crystallin fractions and the occurrence of high-molecular weight material, partly formed by disulphide bonds. The glycated crystallins showed a decrease of tryptophan fluorescence and an increase of a specific non-tryptophan fluorescence. This fluorescence was, however, not exclusively associated with the high molecular weight protein, but was present in all protein fractions. © 1991.
引用
收藏
页码：89 / 94
页数：6
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