PROTEIN METHYLATION IN RAT BRAIN IN VITRO

被引：32

作者：

PAIK, WK

KIM, S

机构：

[1] Pels Research Institute, Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania

来源：

JOURNAL OF NEUROCHEMISTRY | 1969年 / 16卷 / 08期

关键词：

D O I：

10.1111/j.1471-4159.1969.tb05974.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract— Protein‐methylation activity in various organs of the rat was studied with S‐adenosyl‐L‐[methyl‐14C]methionine ([methyl‐14C](SAM) as methyl donor. Activity of the enzyme was highest in brain and lowest in liver. Histones comprised approximately 20 per cent of the total radioactivity incorporated, and lysine‐rich histone was the most active. Analysis of amino acids of the methylated proteins of rat brain showed arginine to be the amino acid most extensively methylated, but some methylation occurred in lysine residues. An additional [methyl‐14C]‐labelled amino acid was found near histidine on the amino acid column chromatogram. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：1257 / &

共 14 条

[1] ACETYLATION + METHYLATION OF HISTONES + THEIR POSSIBLE ROLE IN REGULATION OF RNA SYNTHESIS [J].

ALLFREY, VG ;

FAULKNER, R ;

MIRSKY, AE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (05) :786-+

[2] 3-METHYLHISTIDINE A COMPONENT OF ACTIN [J].

ASATOOR, AM ;

ARMSTRONG, MD .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 26 (02) :168-+

[3] A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].

BRAY, GA .

ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) :279-285

[4]

FIESER LF, 1963, ADVANCED ORGANIC CHE, P493

[5]

Hempel V. K., 1968, HOPPESEYLERS Z PHYSL, V349, P603

[6]

KIM S, 1965, J BIOL CHEM, V240, P4629

[7]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[8]

MOORE BW, 1965, J BIOL CHEM, V240, P1647

[9] OCCURRENCE OF EPSILON-N-METHYL LYSINE IN HISTONES [J].

MURRAY, K .

BIOCHEMISTRY, 1964, 3 (01) :10-+

[10] ENZYMATIC METHYLATION OF PROTEIN FRACTIONS FROM CALF THYMUS NUCLEI [J].

PAIK, WK ;

KIM, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1967, 29 (01) :14-&

← 1 2 →