SPECIFIC INITIATION AT AN ORIGIN OF REPLICATION FROM SCHIZOSACCHAROMYCES-POMBE

被引：45

作者：

CADDLE, MS ^{[1
]}

CALOS, MP ^{[1
]}

机构：

[1] STANFORD UNIV, SCH MED, DEPT GENET, STANFORD, CA 94305 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1994年 / 14卷 / 03期

关键词：

D O I：

10.1128/MCB.14.3.1796

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a genetic assay for efficient autonomous replication, we have isolated from Schizosaccharormyces pombe a 6.2-kb fragment which shows the properties expected of an origin of DNA replication in S. pombe. A 2.8-kb subclone of the fragment has the same replication properties. Two-dimensional gel analysis of replication intermediates throughout plasmids carrying the 6.2- or 2.8-kb fragments shows that replication initiates only in a specific region, which can be localized to within several hundred base pairs, in the fragments. This region is also a site of replication initiation in the S. pombe chromosome where the fragments normally reside. These results provide strong evidence that initiation of replication in S. pombe is localized and mediated by specific DNA sequence signals.

引用

页码：1796 / 1805

页数：10

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