FLOW-INJECTION DETERMINATION OF GLUCOSE, BILE-ACID AND ATP USING IMMOBILIZED ENZYME REACTOR AND CHEMILUMINESCENT ASSAY OF NAD(P)H

We have developed a chemiluminescent flow injection method for analysis of bile acid, glucose and ATP using the chemiluminescent assay of NADH using 1-methoxy-5-methylphenazinium methyl sulphate (1-MPMS)/isoluminol(IL)/microperoxidase (m-POD) system and immobilized enzyme reactors such as 3alpha-hydroxysteroid dehydrogenase, glucosedehydrogenase, hexokinase and glucose-6-phosphate dehydrogenase. The standard curves were obtained in the range of 5 approximately 100 pmol for bile acid, 0.5 approximately 5.0 nmol for glucose and 10(-7) approximately 10(-5)mol/L for ATP. The coefficient of variation for each assay was not more than 4.1% for bile acid, 2.3% for glucose and 5.3% for ATP, respectively.