ISOLATION AND CHARACTERIZATION OF INTRAGENIC SUPPRESSORS OF AN ESCHERICHIA-COLI FTSA MUTATION

被引：5

作者：

ROBINSON, AC ^{[1
]}

BEGG, KJ ^{[1
]}

MACARTHUR, E ^{[1
]}

机构：

[1] ROYAL INFIRM,DEPT MED,EDINBURGH EH3 9HB,MIDLOTHIAN,SCOTLAND

来源：

RESEARCH IN MICROBIOLOGY | 1991年 / 142卷 / 06期

基金：

英国医学研究理事会;

关键词：

MUTATION; CELL DIVISION; ESCHERICHIA-COLI; UV; MUTAGENESIS; TRANSDUCTION; TEMPERATURE; ALLELE FTSA; SUPPRESSION;

D O I：

10.1016/0923-2508(91)90075-L

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutagenesis of a strain of Escherichia coli carrying a temperature-sensitive (Ts) mutation in the cell division gene ftsA yielded a number of temperature-resistant variants. In certain cases, restoration of viability at the restrictive temperature could not be attributed to suppressor mutations occurring in other genes or to structural gene reversion. DNA sequencing of the variants demonstrated the continuing presence of the original Ts mutation (ftsA 13) and revealed secondary mutations within the same gene. These secondary mutations are able to rescue the ftsA 13 mutation in cis, but not in trans.

引用

页码：623 / 631

页数：9

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