A POSTTRANSCRIPTIONAL MECHANISM CONTRIBUTES TO CIRCADIAN CYCLING OF A PER-BETA-GALACTOSIDASE FUSION PROTEIN

被引：46
作者：
ZWIEBEL, LJ
HARDIN, PE
LIU, X
HALL, JC
ROSBASH, M
机构：
[1] BRANDEIS UNIV,HOWARD HUGHES MED INST,WALTHAM,MA 02254
[2] BRANDEIS UNIV,DEPT BIOL,WALTHAM,MA 02254
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 09期
关键词：
GENE EXPRESSION; PERIOD GENE; DROSOPHILA; CIRCADIAN RHYTHMS;
D O I：
10.1073/pnas.88.9.3882
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The period gene (per) of Drosophila melanogaster affects circadian rhythms. Circadian fluctuations in per mRNA levels are thought to contribute to circadian fluctuations in per protein levels in the heads of adult flies. To address the mechanisms underlying these oscillatory phenomena, we have analyzed RNA and protein cycling from two per-beta-galactosidase fusion genes. These studies demonstrate that 5' noncoding sequences from per are sufficient to cause the fusion mRNA levels to cycle in a wild-type (rhythmic) background. Protein cycling requires additional sequences derived from the per coding region. The data suggest that there is a per-dependent post-transcriptional mechanism that is under circadian clock control required for per protein levels to fluctuate in a rhythmic fashion.
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页码：3882 / 3886
页数：5
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