A SURROGATE 15-KDA JC-KAPPA PROTEIN IS EXPRESSED IN COMBINATION WITH MU-HEAVY-CHAIN BY HUMAN B-CELL PRECURSORS

被引：21

作者：

FRANCES, V ^{[1
]}

PANDRAUGARCIA, D ^{[1
]}

GURET, C ^{[1
]}

HO, S ^{[1
]}

WANG, ZX ^{[1
]}

DUVERT, V ^{[1
]}

SAELAND, S ^{[1
]}

MARTINEZVALDEZ, H ^{[1
]}

机构：

[1] SCHERING PLOUGH CORP,IMMUNOL RES LAB,DARDILLY,FRANCE

来源：

EMBO JOURNAL | 1994年 / 13卷 / 24期

关键词：

B LYMPHOPOIESIS; CDNA CLONING; IMMUNOGLOBULIN REARRANGEMENT; GERMLINE KAPPA;

D O I：

10.1002/j.1460-2075.1994.tb06939.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel kappa protein, encoded by a germline JC kappa transcript, is expressed by normal and leukemic human B cell precursors. The transcript displays an open reading frame initiated by a non-AUG codon, and predicts a 15 kDa molecule which could be readily confirmed by in vitro translation. Cellular expression was demonstrated by immunofluorescence. precipitation and Western blotting. Furthermore, 2-D gel electrophoresis revealed that germline JC kappa can covalently associate with Ct heavy chain at the surface of pre-B cells. We therefore propose that during B cell lymphopoiesis, two alternative pathways could be operative in which mu heavy chain can either associate with lambda 5 or germline JC kappa.

引用

页码：5937 / 5943

页数：7

共 44 条

[1] ORGANIZATION AND REORGANIZATION OF IMMUNOGLOBULIN GENES IN A-MULV TRANSFORMED-CELLS - REARRANGEMENT OF HEAVY BUT NOT LIGHT CHAIN GENES [J].