AN OVERVIEW OF THE KINETIC-PARAMETERS OF CLASS-B BETA-LACTAMASES

被引：186

作者：

FELICI, A

AMICOSANTE, G

ORATORE, A

STROM, R

LEDENT, P

JORIS, B

FANUEL, L

FRERE, JM

机构：

[1] UNIV LIEGE,INST CHIM B6,ENZYMOL LAB,B-4000 SART 1,BELGIUM

[2] UNIV LAQUILA,DIPARTIMENTO SCI & TECNOL BIOMED & BIOMETRIA,CATTEDRA CHIM BIOL,I-67100 LAQUILA,ITALY

[3] UNIV ROMA LA SAPIENZA,DIPARTIMENTO BIOPATOL UMANA,CATTEDRA BIOCHIM CLIN,I-00185 ROME,ITALY

来源：

BIOCHEMICAL JOURNAL | 1993年 / 291卷

关键词：

D O I：

10.1042/bj2910151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The catalytic properties of three class B beta-lactamases (from Pseudomonas maltophilia, Aeromonas hydrophila and Bacillus cereus) were studied and compared with those of the Bacteroides fragilis enzyme. The A. hydrophila beta-lactamase exhibited a unique specificity profile and could be considered as a rather specific 'carbapenemase'. No relationships were found between sequence similarities and catalytic properties. The problem of the repartition of class B beta-lactamases into sub-classes is discussed. Improved purification methods were devised for the P. maltophilia and A. hydrophila beta-lactamases including, for the latter enzyme, a very efficient affinity chromatography step on a Zn2+-chelate column.

引用

页码：151 / 155

页数：5

共 36 条

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