ENZYMATIC HYDROLYSIS OF ADENOSINE 3',5'-CYCLIC PHOSPHOROTHIOATE

3′,5′-AMPS (adenosine 3′,5′-cyclic phosphorothioate) does not serve as a substrate or inhibitor of 3′,5′-cyclic nucleotide phosphodiesterase from rat and rabbit brain and from beef heart. Other enzymes, presumably phosphodiesterases, present in the preparations hydrolyze 3′,5′-AMPS with simultaneous displacement of sulfur. Snake venom phosphodiesterases from Crotalus adamanteus and Crotalus terr. terr. hydrolyze 3′,5′-AMPS to 5′-AMP, with adenosine 5′-phosphorothioate (AMPS) as an intermediate. © 1969.