A REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF CLANFENUR IN RAT AND HUMAN PLASMA

被引：2
|
作者：
NOTEBORN, HPJM
VAROSSIEAU, FJ
MONSHOUWER, M
BRANDS, R
PINEDO, HM
机构：
[1] DLO,RIKILT,STATE INST QUAL CONTROL AGR PROD,DEPT RISK ASSESSMENT & TOXICOL,6700 AE WAGENINGEN,NETHERLANDS
[2] SOLVAY DUPHAR BV,DEPT BIOTECHNOL,1380 DA WEESP,NETHERLANDS
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 07期
关键词：
CLANFENUR; REVERSED-PHASE HPLC; BENZOYLPHENYL UREAS; ANTINEOPLASTIC DRUGS;
D O I：
10.1016/0731-7085(94)E0013-Q
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A selective and specific high-pressure liquid chromatographic (HPLC) method for the simultaneous assay of Clanfenur and its metabolites in biological fluids of interest has been developed which is suitable for routine analysis, using micro volumes (0.1 ml) of plasma samples only. After protein precipitation the extract is analysed by reversed-phase HPLC with UV detection. Excellent recovery, linearity, accuracy and precision (less than 5% for plasma) are achieved by the assay which is able to quantify Clanfenur and its metabolites in plasma at concentrations between 0.025 and 5.0 mg l-1.
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页码：937 / 942
页数：6
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