PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF RAT AND HUMAN STEM-CELL FACTOR DNAS

被引:785
作者
MARTIN, FH
SUGGS, SV
LANGLEY, KE
LU, HS
TING, J
OKINO, KH
MORRIS, CF
MCNIECE, IK
JACOBSEN, FW
MENDIAZ, EA
BIRKETT, NC
SMITH, KA
JOHNSON, MJ
PARKER, VP
FLORES, JC
PATEL, AC
FISHER, EF
ERJAVEC, HO
HERRERA, CJ
WYPYCH, J
SACHDEV, RK
POPE, JA
LESLIE, I
WEN, DZ
LIN, CH
CUPPLES, RL
ZSEBO, KM
机构
[1] AMGEN Inc. AMGEN Center Thousand Oaks
关键词
D O I
10.1016/0092-8674(90)90301-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Partial cDNA and genomic clones of rat stem cell factor (SCF) have been isolated. Using probes based on the rat sequence, partial and full-length cDNA and genomic clones of human SCF have been isolated. Based on the primary structure of the 164 amino acid protein purified from BRL-3A cells, truncated forms of the rat and human proteins have been expressed in E. coli and mammalian cells and have been shown to possess biological activity. SCF is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF exhibits potent synergistic activities in conjunction with colony-stimulating factors, resulting in increased colony numbers and colony size. © 1990.
引用
收藏
页码:203 / 211
页数:9
相关论文
共 53 条
[1]   CONTROLLED SYNTHESIS OF HBSAG IN A DIFFERENTIATED HUMAN-LIVER CARCINOMA-DERIVED CELL-LINE [J].
ADEN, DP ;
FOGEL, A ;
PLOTKIN, S ;
DAMJANOV, I ;
KNOWLES, BB .
NATURE, 1979, 282 (5739) :615-616
[2]  
ALTUS MS, 1971, P SOC EXP BIOL MED, V138, P985
[3]  
BRADLEY TR, 1979, BLOOD, V54, P1446
[4]   DIRECT EXPRESSION OF BORDETELLA-PERTUSSIS TOXIN SUBUNITS TO HIGH-LEVELS IN ESCHERICHIA-COLI [J].
BURNETTE, WN ;
MAR, VL ;
CIEPLAK, W ;
MORRIS, CF ;
KALJOT, KT ;
MARCHITTO, KS ;
SACHDEV, RK ;
LOCHT, C ;
KEITH, JM .
BIO-TECHNOLOGY, 1988, 6 (06) :699-706
[5]  
CHANG JY, 1989, TECHNIQUES PROTEIN C, P305
[6]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[7]   SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].
CHEN, EY ;
SEEBURG, PH .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170
[8]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[9]  
Coffman R L, 1982, Immunol Rev, V69, P5
[10]  
COON HG, 1968, J CELL BIOL, V39, pA29