THE USE OF DIPHENYLENE IODONIUM, AN INHIBITOR OF NADPH OXIDASE, TO INVESTIGATE THE ANTIMICROBIAL ACTION OF HUMAN MONOCYTE DERIVED MACROPHAGES

被引：28

作者：

ROBERTSON, AK

CROSS, AR

JONES, OTG

ANDREW, PW

机构：

[1] UNIV LEICESTER,DEPT MICROBIOL,UNIV RD,LEICESTER LE1 9HN,ENGLAND

[2] UNIV BRISTOL,DEPT BIOCHEM,BRISTOL BS8 1TD,AVON,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 133卷 / 02期

关键词：

Antimicrobial activity; Diphenylene iodonium; Hydrogen peroxide; Macrophage; Toxoplasma;

D O I：

10.1016/0022-1759(90)90357-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Diphenylene iodonium is an inhibitor of the respiratory burst-generating NADPH oxidase of phagocytes. The effect of this compound on human monocyte-derived macrophages and its usefulness in exploring the antimicrobial mechanisms of phagocytes was examined. 1 μM diphenylene iodonium inhibited hydrogen peroxide production by human macrophages and the activity of these cells against Toxoplasma gondii. At this concentration macrophage degranulation was unaffected. © 1990.

引用

页码：175 / 179

页数：5

共 10 条

[1]

ANDREW PW, 1985, MONONUCLEAR PHAGOCYT, P311

[2] THE EFFECT OF THE INHIBITOR DIPHENYLENE IODONIUM ON THE SUPEROXIDE-GENERATING SYSTEM OF NEUTROPHILS - SPECIFIC LABELING OF A COMPONENT POLYPEPTIDE OF THE OXIDASE [J].