SCREENING FOR AMINES BY DANSYLATION AND AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：35
作者：
PRICE, NPJ
FIRMIN, JL
GRAY, DO
机构：
[1] UNIV LONDON,QUEEN MARY & WESTFIELD COLL,SCH BIOL SCI,LONDON E1 4NS,ENGLAND
[2] JOHN INNES INST,JOHN INNES CTR PLANT SCI RES,NORWICH NR4 7UH,NORFOLK,ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 598卷 / 01期
关键词：
D O I：
10.1016/0021-9673(92)85113-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A reversed-phase high-performance liquid chromatographic procedure for the determination of a broad range of amines after derivatization with dansyl chloride is presented. Forty-five amines were tested over the concentration range 2-16-mu-M. Linear calibration graphs passing through the origin were obtained with 25 amines. The method was applied to the identification of biogenic amines from Agrobacterium-transformed plant tissue. Reliability and ease of operation make the procedure particularly well suited to automation, permitting the rapid analysis of a large number of samples.
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页码：51 / 57
页数：7
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