AFFINITY CHROMATOGRAPHIC PURIFICATION OF BOVINE LUNG ENDOTHELIN RECEPTOR USING BIOTINYLATED ENDOTHELIN AND AVIDIN AGAROSE

被引：12
作者：
HAGIWARA, H
NAGASAWA, T
LODHI, KM
KOZUKA, M
ITO, T
HIROSE, S
机构：
[1] Department of Biological Sciences, Tokyo Institute of Technology, Meguroku, Tokyo, 152, Ookayama
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 597卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(92)80128-H
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Endothelin receptor was purified from bovine lung by affinity chromatography using biotinylated endothelin and avidin agarose. Endothelin was biotinylated with sulphosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate, a reactive form of biotin with a cleavable spacer arm containing a disulphide bond designed for a simple elution by thiols. Starting from 3.5 kg of bovine lung, about 200-mu-g of pure receptor were obtained.
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页码：331 / 334
页数：4
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