CHARACTERIZATION OF MONOCLONAL-ANTIBODIES REACTIVE WITH NORMAL RESTING, LACTATING AND NEOPLASTIC HUMAN BREAST

被引:4
作者
SKILTON, RA
EARL, HM
GORE, ME
MCILHINNEY, RAJ
GUSTERSON, BA
WILSON, P
COOMBES, RC
NEVILLE, AM
机构
[1] ST GEORGE HOSP,SCH MED,DEPT MED ONCOL,CRANMER TERRACE,LONDON SW17 0RE,ENGLAND
[2] LUDWIG INST CANC RES,SUTTON,SURREY,ENGLAND
关键词
Breast cancer; Epithelial markers; Human milk; Monoclonal antibodies; Myoepithelial markers;
D O I
10.1159/000217640
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Mouse monoclonal antibodies reacting with human mammary gland constituents have been generated and characterized in an attempt to raise breast- or breast-cancer-specific antisera. The immunogens used in these studies included fractionated human milk fat globule membrane, the human breast cancer cell line MCF7 and a crude membrane preparation derived from an axillary nodal metastasis from a patient with breast cancer. Of the antibodies obtained, 8 were characterized and found to bind to different structures in the normal breast. The antibody L1CR-LON-LC28 recognizes secretory component and binds strongly to normal resting and lactating breast, but only focally to a minority of breast carcinomas. The antibodies L1CR-LON-14.1 and 32.2 react strongly with the lactating breast and recognize κ- and β-casein, respectively. Caseins are not produced by breast tumors. The antibodies L1CR-LON-TW19.5, H10A and 39.8 all react with carbohydrate epitopes and bind heterogeneously to normal resting breast luminal epithelium and cellular subsets of breast carcinomas. LICR-LON-59.2 and 19.2 react with normal breast myoepithelial cells and the basement membrane, respectively. LICR-LON-59.2 is unusual as a myoepithelial marker in that it stains cells in the majority of breast carcinomas. LICR-LON-19.2 shows extensive reactivity to tumor cell lines in culture but has no reactivity with carcinoma cells from breast biopsies. © 1990 S. Karger AG, Basel.
引用
收藏
页码:20 / 38
页数:19
相关论文
共 50 条
[21]   THE PEPTIDASE ACTIVITY OF HUMAN SERUM BUTYRYLCHOLINESTERASE - STUDIES USING MONOCLONAL-ANTIBODIES AND CHARACTERIZATION OF THE PEPTIDASE [J].
RAO, RV ;
BALASUBRAMANIAN, AS .
JOURNAL OF PROTEIN CHEMISTRY, 1993, 12 (01) :103-110
[22]   PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST A HUMAN OVARIAN TERATOCARCINOMA CELL-LINE [J].
LEMIEUX, P ;
PAGE, M .
ANTICANCER RESEARCH, 1994, 14 (6B) :2709-2715
[23]   RADIOIMMUNODETECTION OF HUMAN GLIOMA XENOGRAFTS BY RADIOLABELED MONOCLONAL-ANTIBODIES [J].
STAVROU, D ;
FREIBERG, B ;
MEYERMANN, R ;
ANZIL, AP ;
KEIDITSCH, E ;
MEHRAEIN, P .
ANTICANCER RESEARCH, 1991, 11 (02) :537-542
[24]   PRIMATES MONOCLONAL-ANTIBODIES - THEIR POTENTIAL ADVANTAGES IN HUMAN THERAPY [J].
BLANCHER, A ;
ROUBINET, F ;
BENARD, P .
REVUE DE MEDECINE VETERINAIRE, 1994, 145 (06) :419-431
[25]   MONOCLONAL-ANTIBODIES THAT RECOGNIZE THE NATIVE HUMAN THYROTROPIN RECEPTOR [J].
JOHNSTONE, AP ;
CRIDLAND, JC ;
DACOSTA, CR ;
HARFST, E ;
SHEPHERD, PS .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1994, 105 (02) :R1-R9
[26]   MONOCLONAL-ANTIBODIES AGAINST ANTIGENS EXPRESSED BY HUMAN SPERM [J].
ZIEGLER, A ;
HIRSCH, T ;
KRAUSE, W ;
NEUMANN, K ;
SCHIEFERSTEIN, G ;
DOHR, G ;
KOHLSTADT, S ;
UCHANSKAZIEGLER, B .
ANDROLOGIA, 1990, 22 :101-109
[27]   IDENTIFICATION OF STRUCTURALLY ALTERED ESTROGEN-RECEPTORS IN HUMAN BREAST-CANCER BY SITE-DIRECTED MONOCLONAL-ANTIBODIES [J].
TRAISH, AM ;
ALFADHLI, S ;
KLINGE, C ;
KOUNINE, M ;
QUICK, TC .
STEROIDS, 1995, 60 (07) :467-474
[28]   PREPARATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO SEROTONIN BINDING-PROTEIN [J].
LIU, KP ;
YU, PY ;
HSIUNG, SH ;
KIRCHGESSNER, AL ;
GERSHON, MD ;
TAMIR, H .
JOURNAL OF NEUROCHEMISTRY, 1990, 55 (03) :1013-1021
[29]   CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO CONIDIAL ANTIGENS OF PYRICULARIA-GRISEA [J].
XIA, JQ ;
LEE, FN ;
KIM, KS ;
SPIEGEL, FW .
EXPERIMENTAL MYCOLOGY, 1993, 17 (03) :170-181
[30]   CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO TOMATO ASPERMY VIRUS AND THEIR USE IN DIAGNOSTICS [J].
AMBROSOVA, SM ;
MALOFEYEVA, YS ;
DZIRKALE, LT .
BIOORGANICHESKAYA KHIMIYA, 1994, 20 (11) :1169-1174