ASSIGNMENT OF 2 RAT DIHYDROFOLATE (DHFR) GENES TO CHROMOSOME-2 AND CHROMOSOME-4

被引：4

作者：

HANSON, C ^{[1
]}

ANDERSSON, A ^{[1
]}

ISLAM, MQ ^{[1
]}

SZPIRER, J ^{[1
]}

SZPIRER, C ^{[1
]}

LEVAN, G ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,DEPT BIOL MOLEC,B-1050 BRUSSELS,BELGIUM

来源：

CYTOGENETICS AND CELL GENETICS | 1990年 / 53卷 / 01期

关键词：

D O I：

10.1159/000132888

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A panel of rat × mouse cell hybrids was used in the chromosomal mapping of the rat dihydrofolale reductase (DHFR) gene. It was determined that the probe hybridized to gene sequences on two different chromosomes (Nos. 2 and 4). possibly representing the active gene and a pseudogene. Hybridization of the DHFR probe to DNA from a methotrexate resistant rat cell line revealed that the gene on chromosome 2 was amplified, but not the gene on chromosome 4. This result was taken to suggest that the active DHFR gene is located on rat chromosome 2 and that the sequence on chromosome 4 is a pseudogene. © 1990 S. Karger AG, Basel.

引用

页码：23 / 25

页数：3

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