A HIGHLY SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR CLOSTRIDIUM-PERFRINGENS ENTEROTOXIN

被引：8
|
作者：
UEMURA, T
YOSHITAKE, S
HU, D
KAJIKAWA, T
机构：
[1] Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Sakai-Shi, Osaka, 591
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1992年 / 15卷 / 01期
关键词：
D O I：
10.1111/j.1472-765X.1992.tb00714.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A highly sensitive enzyme-linked immunosorbent assay (ELISA) for quantitation of Clostridium perfringens enterotoxin (CPE) by a sandwich method with polystyrene beads was elaborated. The ELISA was very sensitive with a detection limit of 1 pg/ml of CPE. Clostridium perfringens culture fluid did not interfere with the assay. This ELISA may be useful for the mass screening for Cl. perfringens producing small amounts of CPE.
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页码：23 / 25
页数：3
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