KINETIC CHARACTERIZATION OF CHIMERIC CYCLOMALTODEXTRIN GLUCANOTRANSFERASE FROM GENES OF 2 ALKALOPHILIC BACILLUS

The chimeric CGTase, which was constructed genetically with two β‐CD forming type enzyme genes of different species of alkalophilic Bacillus, was characterized by comparing with the actions of parent enzymes. The final composition of CDs in the reaction digest from starch by the chimeric enzyme, especially in the yield of α‐CD, was different from those by parent enzymes, and total yields of CDs by these enzymes were almost the same. The chimeric enzyme catalyzed the formation of β‐CD as a major action from starch, the rapid synthesis of α‐CD from other CDs and maltooligosaccharides and the slow degradation and interconversion of α‐CD to others. From these results, the role of second and third segments in the enzyme molecules was discussed. Copyright © 1990 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim