PROPERTIES OF CYANOGEN BROMIDE-ACTIVATED, AGAROSE-IMMOBILIZED CATECHOL 1,2-DIOXYGENASE FROM FREEZE-DRIED EXTRACTS OF NOCARDIA SP NCIB-10503

被引：8

作者：

SMITH, MR

RATLEDGE, C

CROOK, S

机构：

[1] CASTROL RES LABS, READING, ENGLAND

[2] UNIV HULL, DEPT BIOCHEM, HULL HU6 7RX, N HUMBERSIDE, ENGLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 12期

关键词：

activated Agarose; Catechol 1,2-dioxygenase; immobilized enzyme;

D O I：

10.1016/0141-0229(90)90114-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Catechol 1,2-dioxygenase [catechol: oxygen 1,2-oxidoreductase (decyclizing); EC 1.13.11.1], the aromatic intradiol ring-cleaving enzyme of Nocardia sp. NCIB 10503 prepared by freeze-drying cell-free extracts, was covalently attached to cyanogen bromide-activated Agarose. The properties of the immobilized enzyme were compared to those of the free enzyme preparation. Immobilization was shown to increase the thermal stability of the enzyme. The pH-activity profile was altered by immobilization. Various explanations for this phenomenon are discussed. The Vmax and Km of the enzyme were not significantly affected on immobilization. The enzyme had a broader substrate specificity than any previously reported catechol 1,2-dioxygenase, and this was largely unaltered by immobilization. The properties of the preparations are compared to those of other (free) catechol 1,2-dioxygenases. The results presented show that the immobilization of catechol 1,2-dioxygenase offers an attractive means for the production of cis,cis-muconate and novel substituted analogues. © 1990.

引用

页码：945 / 949

页数：5

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