PLASMA-CLEARANCE, ORGAN DISTRIBUTION AND TARGET-CELLS OF INTERLEUKIN-6 HEPATOCYTE-STIMULATING FACTOR IN THE RAT

被引：302
作者：
CASTELL, JV
GEIGER, T
GROSS, V
ANDUS, T
WALTER, E
HIRANO, T
KISHIMOTO, T
HEINRICH, PC
机构：
[1] UNIV FREIBURG, INST BIOCHEM, HERMANN HERDER STR 7, D-7800 FREIBURG, FED REP GER
[2] UNIV FREIBURG, MED KLIN 1, D-7800 FREIBURG, FED REP GER
[3] OSAKA UNIV, INST MOLEC & CELLULAR BIOL, OSAKA, JAPAN
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1988年 / 177卷 / 02期
关键词：
D O I：
10.1111/j.1432-1033.1988.tb14384.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The plasma half-life of recombinant human interleukin-6 (rhIL-6) was determined in rats by measuring the disappearance of the biological activity as well as of the radioactivity of 125I-rhIL-6 from the circulation. The kinetics of clearance were biphasic. It consisted of a rapid initial disappearance corresponding to a half-life of 3 min, and of a second slow one corresponding to a half-life of about 55 min. By cellulose-acetate electrophoresis it was shown that rhIL-6 binds to a plasma protein resulting in a complex migrating in the .beta.-.gamma. region; 20 min after intravenous injection, about 80% of the 125I-rhIL-6 that had disappeared from the circulation was found in the liver. 125I-rhIL-6 was exclusively localized on the surface of parenchymal cells suggesting the existence of an interleukin-6 receptor on the hepatocytes.
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页码：357 / 361
页数：5
相关论文
共 16 条
[1]   REGULATION OF SYNTHESIS AND SECRETION OF MAJOR RAT ACUTE-PHASE PROTEINS BY RECOMBINANT HUMAN INTERLEUKIN-6 (BSF-2/IL-6) IN HEPATOCYTE PRIMARY CULTURES [J].
ANDUS, T ;
GEIGER, T ;
HIRANO, T ;
KISHIMOTO, T ;
TRANTHI, TA ;
DECKER, K ;
HEINRICH, PC .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 173 (02) :287-293
[2]   RECOMBINANT HUMAN B-CELL STIMULATORY FACTOR-II (BSF-2/IFN-BETA2) REGULATES BETA-FIBRINOGEN AND ALBUMIN MESSENGER-RNA LEVELS IN FAO-9 CELLS [J].
ANDUS, T ;
GEIGER, T ;
HIRANO, T ;
NORTHOFF, H ;
GANTER, U ;
BAUER, J ;
KISHIMOTO, T ;
HEINRICH, PC .
FEBS LETTERS, 1987, 221 (01) :18-22
[3]   RECOMBINANT HUMAN INTERLEUKIN-6 (IL-6/BSF-2/HSF) REGULATES THE SYNTHESIS OF ACUTE PHASE PROTEINS IN HUMAN HEPATOCYTES [J].
CASTELL, JV ;
GOMEZLECHON, MJ ;
DAVID, M ;
HIRANO, T ;
KISHIMOTO, T ;
HEINRICH, PC .
FEBS LETTERS, 1988, 232 (02) :347-350
[4]   HIGH-AFFINITY BINDING-SITES FOR HUMAN 26-KDA PROTEIN (INTERLEUKIN 6, B-CELL STIMULATORY FACTOR-II, HUMAN HYBRIDOMA PLASMACYTOMA GROWTH-FACTOR, INTERFERON-BETA-2), DIFFERENT FROM THOSE OF TYPE-I INTERFERON (ALPHA,BETA), ON LYMPHOBLASTOID-CELLS [J].
COULIE, PG ;
VANHECKE, A ;
VANDAMME, J ;
CAYPHAS, S ;
POUPART, P ;
DEWIT, L ;
CONTENT, J .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1987, 17 (10) :1435-1440
[5]  
DESCHATRETTE J, 1974, BIOCHIMIE, V56, P1603
[6]   INTERFERON BETA-2/B-CELL STIMULATORY FACTOR TYPE-2 SHARES IDENTITY WITH MONOCYTE-DERIVED HEPATOCYTE-STIMULATING FACTOR AND REGULATES THE MAJOR ACUTE PHASE PROTEIN RESPONSE IN LIVER-CELLS [J].
GAULDIE, J ;
RICHARDS, C ;
HARNISH, D ;
LANSDORP, P ;
BAUMANN, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :7251-7255
[7]   INDUCTION OF RAT ACUTE-PHASE PROTEINS BY INTERLEUKIN-6 INVIVO [J].
GEIGER, T ;
ANDUS, T ;
KLAPPROTH, J ;
HIRANO, T ;
KISHIMOTO, T ;
HEINRICH, PC .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (05) :717-721
[8]   COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN [J].
HIRANO, T ;
YASUKAWA, K ;
HARADA, H ;
TAGA, T ;
WATANABE, Y ;
MATSUDA, T ;
KASHIWAMURA, S ;
NAKAJIMA, K ;
KOYAMA, K ;
IWAMATSU, A ;
TSUNASAWA, S ;
SAKIYAMA, F ;
MATSUI, H ;
TAKAHARA, Y ;
TANIGUCHI, T ;
KISHIMOTO, T .
NATURE, 1986, 324 (6092) :73-76
[9]   PURIFICATION TO HOMOGENEITY AND CHARACTERIZATION OF HUMAN B-CELL DIFFERENTIATION FACTOR (BCDF OR BSFP-2) [J].
HIRANO, T ;
TAGA, T ;
NAKANO, N ;
YASUKAWA, K ;
KASHIWAMURA, S ;
SHIMIZU, K ;
NAKAJIMA, K ;
PYUN, KH ;
KISHIMOTO, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (16) :5490-5494
[10]   ABSENCE OF ANTIVIRAL ACTIVITY IN RECOMBINANT B-CELL STIMULATORY FACTOR-II (BSF-2) [J].
HIRANO, T ;
MATSUDA, T ;
HOSOI, K ;
OKANO, A ;
MATSUI, H ;
KISHIMOTO, T .
IMMUNOLOGY LETTERS, 1988, 17 (01) :41-45
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