A COMPARISON OF HUMAN AND MURINE MONOCLONAL IGGS SPECIFIC FOR THE P1.7 PORA PROTEIN OF NEISSERIA-MENINGITIDIS

被引：17

作者：

DELVIG, A

JAHN, S

KUSECEK, B

HECKELS, JE

ROSENQVIST, E

HOIBY, EA

MICHAELSEN, TE

ACHTMAN, M

机构：

[1] MAX PLANCK INST MOLEC GENET,D-14195 BERLIN,GERMANY

[2] HUMBOLDT UNIV BERLIN,INST MED IMMUNOL CHARITE,D-10117 BERLIN,GERMANY

[3] UNIV SOUTHAMPTON,SOUTHAMPTON GEN HOSP,SOUTH LAB,DEPT MICROBIOL,SOUTHAMPTON SO9 4XY,HANTS,ENGLAND

[4] NATL INST PUBL HLTH,DEPT VACCINE,N-0462 OSLO 4,NORWAY

[5] NATL INST PUBL HLTH,DEPT BACTERIOL,N-0462 OSLO 4,NORWAY

来源：

MOLECULAR IMMUNOLOGY | 1994年 / 31卷 / 16期

关键词：

MONOCLONAL HUMAN IGG; PORIN; PROTECTION; VACCINE DEVELOPMENT; LINEAR EPITOPES;

D O I：

10.1016/0161-5890(94)90076-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monoclonal human IgG SS269 reacts with Neisseria meningitidis expressing the P1.7 PorA protein and with linear peptides containing NGGAS, which accounts for the P1.7 specificity. Murine monoclonal antibody to P1.7 reacts with peptides containing the overlapping epitope, ASGQ. The human and murine antibodies have similar affinities. The low avidity human antibody is very inefficient at stimulating complement-mediated bactericidal killing while the high avidity murine antibody efficiently kills bacteria. However, efficient opsonophagocytosis was mediated even at low concentrations of the human body antibody and in the absence of complement, suggesting that low avidity antibodies might be protective against disease.

引用

页码：1257 / 1267

页数：11

共 62 条

[1] THE EXTENDED HINGE REGION OF IGG3 IS NOT REQUIRED FOR HIGH PHAGOCYTIC CAPACITY MEDIATED BY FC-GAMMA-RECEPTORS, BUT THE HEAVY-CHAINS MUST BE DISULFIDE-BONDED [J].