DETECTION OF CANDIDA-ALBICANS AND OTHER YEASTS IN BLOOD BY PCR

被引：89
|
作者：
HOLMES, AR
CANNON, RD
SHEPHERD, MG
JENKINSON, HF
机构：
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1994年 / 32卷 / 01期
关键词：
D O I：
10.1128/JCM.32.1.228-231.1994
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Primers complementary to the region of genes coding for rRNA in Candida albicans were used in PCRs to detect yeast DNA extracted from blood samples containing various Candida specie$. One fragment (105 bp) was amplified from all yeasts tested, whereas a second (684 bp) was only amplified when C. albicans DNA was present. The level of sensitivity was 15 +/- 5 (mean +/- standard error) CFU of C. albicans per ml of blood.
引用
收藏
页码：228 / 231
页数：4
相关论文
共 50 条
12345